I stained pancreatic cancer cells (Panc-1) for gamma-H2AX and got a large amount of foci in control cells (cells which have not undergone any genotoxic stress).

Developmental effects of fractionated low-dose exposure to gamma radiation on Long time persistence of residual 53BP1/gamma-H2AX foci in human 

I stained pancreatic cancer cells (Panc-1) for gamma-H2AX and got a large amount of foci in control cells (cells which have not undergone any genotoxic stress). Irradiation-independent nuclear γ-H2AX staining in leptotene spermatocytes demonstrates a function for γ-H2AX during meiosis. γ-H2AX staining in intermediate and B spermatogonia, preleptotene spermatocytes, and sex vesicles and round spermatids, however, indicates that the function of H2AX phosphorylation during spermatogenesis is not restricted to the formation of γ-H2AX foci at DNA double-strand breaks. Staining of γ-H2AX foci in blood lymphocytes is based upon phosphorylation of the histone variant H2AX after formation of DSBs.

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Incubate sections in 3% hydrogen peroxide for 10 min. Wash sections in dH 2 O two times for 5 min each. Wash sections in wash buffer for 5 min. Block each section with 100–400 µl of preferred blocking solution for 1 hr at room temperature. Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Histone Gamma-H2A.X (Phospho S139) Clone RM224 quantity Add to cart SKU: 31-1104-00 Categories: Histone H2A.Z Acetylation , Histone Modification , Primary Antibodies , Rabbit Monoclonal Tags: 31-1104-00 , 31-1104-00-S , 31110400 , 31110400S Simple Western: gamma H2AX [p Ser139] Antibody (3F2) [NB100-74435] - Electropherogram image(s) of corresponding Simple Western lane view. Gamma H2AX antibody was used at 10 ug/ml dilution on Jurkat lysate(s). Flow cytometric analysis of HeLa cells, untreated (blue) or treated with UV (100mJ/cm2, 2 hr recovery; green) using Phospho-Histone H2A.X (Ser139) Antibody (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed lines).

γ-H2AX staining was not attributable to host DNA damage, as the host genome was found to be intact. The response was rather a result of overactivated ATR, 

Toxicology Letters , 314 , S122-S122. BioAssay record AID 1254631 submitted by ChEMBL: Induction of DNA damage in human HCT116 cells at G0/G1 phase at 30 uM after 24 hrs by gamma-H2AX-staining based-flow cytometry. Gamma H2A.X Staining Kit (ab242296) is based on the phosphorylation of the histone H2A.X at serine 139 in response to DNA damaging agents which cause double strand breaks in cells that are cultured in microtiter plates. The kit provides sufficient reagents for up to 100 stainings in 96- well plate Phosphorylation of the chromatin protein H2AX (forming γH2AX) is implicated in the repair of DNA double strand breaks (DSB's); a large number of H2AX molecules become phosphorylated at the sites of DSB's.

and Therapy 2012:5 309–314 OncoTargets and Therapy γ-H2AX expression (C) Immunohistochemistry staining of BIRC6 in tumor tissues and self-paired 

(C-E) Bar graphs showing quantification of γ-H2AX IFA staining (C), % γ-H2AX ELISA readings (D), and PAR ELISA readings (E) in xenograft tumors.

As such, H2AX (pS139) immunofluorescence localization serves as a biomarker for nuclear sites of DNA damage (e.g., double-stranded DNA breaks) in affected cells. Flow cytometric analysis of H2AX (pS139) expression in 2005-07-19 · However, the most intensely staining γ-H2AX foci (i.e., the large γ-H2AX foci) were frequently observed juxtaposed to, rather than superimposed over, highly compacted heterochromatin as identified by the sharp peaks in the DAPI channel (Figure 1; our unpublished data).
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Moreover, since gamma‐H2AX staining can be performed on formalin‐fixed and paraffin‐embedded tissue sections generated during repeated‐dose toxicity studies, it does not require any further treatments or extra procedures during dissection, thus optimizing the use of resources and animals.

(C-E) Bar graphs showing quantification of γ-H2AX IFA staining (C), % γ-H2AX ELISA readings (D), and PAR ELISA readings (E) in xenograft tumors. Mice were treated as indicated on the bottom of each figure. Abstract UV irradiation induces histone variant H2AX phosphorylated on serine 139 (γH2AX) foci and high levels of pan-nuclear γH2AX staining without foci, but the significance of this finding is still uncertain. We examined the formation of γH2AX and 53BP1 that coincide at sites of double-strand breaks (DSBs) after ionizing radiation.
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Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4).

15 May 2019 further measurement of the γ-H2AX fluorescence intensity and foci formation ( Figure 2D). Pan-.